Lab members are highlited in bold
Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Tung Le* (2020) Diversification of DNA-binding specificity via permissive and specificity-switching mutations in the ParB/Noc protein family. Cell Reports. 32(3):107928 (PREPRINT version). (PDB:6S6H and 6Y93) (GEO)
Jalal ASB, Tung Le* (2020) Bacterial chromosome segregation by the ParABS system. Open Biology. 10: 200097.
Jalal ASB, Tran NT, Tung Le* (2020) ParB spreading on DNA requires cytidine triphosphate in vitro. eLIFE 9:e83515. (PREPRINT version)
Jalal ASB, Pastrana CL, Tran NT, Stevenson CE, Lawson DM, Moreno-Herrero F, Tung Le (2019) Structural and biochemical analyses of Caulobacter crescentus ParB reveal the role of its N-terminal domain in chromosome segregation. PREPRINT. DOI. (PDB: 6T1F) (GEO)
Tung Le* (2019) Chromosome conformation capture with deep sequencing to study the roles of the Structural Maintenance of Chromosomes (SMC) complex in vivo. Methods in Molecular Biology 2004:105-118. (FREE version)
Schumacher MA, den Hengst CD, Bush MJ, Tung Le, Tran NT, Chandra G, Zeng W, Travis B, Brennan RG, Buttner MJ (2018) The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.Nature Communications 9(1):1139-1151
Tran NT, Stevenson CE, Som NF, Thanapipatsiri A, Jalal ASB, Tung Le* (2018) Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome. Nucleic Acids Research. 46 (3), 1196–1209. (PREPRINT version) (GEO)
Tran NT, Laub MT, Tung Le* (2017) SMC progressively aligns chromosomal arms in Caulobacter crescentus but is antagonized by convergent transcription. Cell Reports 20(9):2057-2071 (PREPRINT version) (GEO)
Badrinarayanan A, Tung Le, Spille JH, Cisse II, Laub MT. (2017) Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB. PLoS Genetics. 13(5): e1006783. (GEO)
Wang X, Brandão HB, Tung Le, Laub MT, Rudner DZ (2017) Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus. Science 355 (6324): 524-527
Tung Le and Laub MT (2016) Transcription rate and transcript length drive chromosomal interaction domain boundaries. EMBO Journal. 35(14):1582-95 (GEO)
Wang X, Tung Le, Lajoie BR, Dekker J, Laub MT, Rudner DZ (2015) Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtillus. Genes and Development 29(15):1661–75
Badrinarayanan A., Tung Le, Laub MT (2015) Rapid pairing and subsequent resegregation of distant homologous loci enables double-strand break repair in bacteria. Journal of Cell Biology 210(3):385–400 (GEO)
Schäfer M, Tung Le, Hearnshaw SJ, Maxwell A, Challis GL, Wilkinson B, Buttner MJ (2015) SimC7 Is a Novel NAD(P)H-Dependent Ketoreductase Essential for the Antibiotic Activity of the DNA Gyrase Inhibitor Simocyclinone. Journal of Molecular Biology. 427(12): 2192-204
Badrinarayanan A†, Tung Le†, Laub MT (2015) Bacterial chromosome organization and segregation. Annual Review of Cell and Developmental Biology 31:171-99 († co-first)
Tung Le and Laub MT (2014) New approaches to understanding the spatial organization of bacterial genomes. Current Opinion in Microbiology 22:15-21
Tung Le, Imakaev MV, Mirny LA, Laub MT (2013) High-resolution mapping of the spatial organization of a bacterial chromosome. Science 342: 731-734 (GEO)
Stevenson CE, Assaad A, Chandra G, Tung Le, Greive SJ, Bibb MJ, Lawson DM (2013) Investigation of DNA sequence recognition by a streptomycete MarR family transcriptional regulator through surface plasmon resonance and X-ray crystallography. Nucleic Acids Research 41: 7009-7022
Tung Le*, Schumacher MA, Lawson DM, Brennan RG, Buttner MJ (2011) The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding. Nucleic Acids Research 39: 9433-9447.
Tung Le, Stevenson CE, Fiedler HP, Maxwell A, Lawson DM, Buttner MJ (2011) Structures of the TetR-like simocyclinone efflux pump repressor, SimR, and the mechanism of ligand-mediated derepression. Journal of Molecular Biology 408: 40-56.
Tung Le, Stevenson CE, Buttner MJ, Lawson DM (2011) Crystallization and preliminary X-ray analysis of the TetR-like efflux pump regulator SimR. Acta Crystallogr Sect F Struct Biol Cryst Commun 67: 307-309.
Edwards MJ, Flatman RH, Mitchenall LA, Stevenson CE, Tung Le, Clarke TA, McKay AR, Fiedler HP, Buttner MJ, Lawson DM, Maxwell A (2009) A crystal structure of the bifunctional antibiotic simocyclinone D8, bound to DNA gyrase. Science 326: 1415-1418.
Tung Le*, Fiedler HP, den Hengst CD, Ahn SK, Maxwell A, Buttner MJ (2009) Coupling of the biosynthesis and export of the DNA gyrase inhibitor simocyclinone in Streptomyces antibioticus. Mol Microbiol 72: 1462-1474.