*corresponding author
Lab members are highlighted in bold
2024
Kamat A, Tran N, Sharda M, Sontakke N, Tung Le*, Badrinarayanan A*. (2024). Widespread prevalence of a post-translational modification in activation of an essential bacterial DNA damage response.PLOS Biology 22:e3002540. doi:10.1371/journal.pbio.3002540 (preprint version)
McLean TC*, Balaguer-Perez F, Chandanani J, Thomas CM, Aicart-Ramos C, Burick S, Olinares PDB, Gobbato G, Mundy JEA, Chait BT, Lawson DM, Darst SA*, Campbell EA*, Moreno-Herrero F*, Tung Le*. (2024). Molecular switching of a DNA-sliding clamp to a repressor mediates long-range gene silencing. BioRxiv doi:10.1101/2024.02.16.579611
Banks EJ*, Tung Le*. (2024) Co-opting bacterial viruses for DNA exchange: structure and regulation of gene transfer agents. Current Opinion in Microbiology 2024, 78:10243. https://doi.org/10.1016/j.mib.2024.102431
McLean TC* (2024) LazyAF, a pipeline for accessible medium-scale in silico prediction of protein-protein interactions. BioRxiv 2024.01.29.577767 doi:10.1101/2024.01.29.577767 (useful new tool)
2023
Tran NT, Tung Le*. (2023) Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination. BioRxiv, doi:10.1101/2023.11.12.565669.
Tišma M, Kaljević J, Gruber S, Tung Le, Dekker C*. (2023) Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies. FEMS Microbiology Reviews https://doi.org/10.1093/femsre/fuad067
Kaljević J, Tesseur C, Tung Le, Laloux G* . (2023). Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system. PLOS Genetics 19:e1010951. doi:10.1371/journal.pgen.1010951 (preprint version)
Sukhoverkov KV, Jalal ASB, Ault J, Sobott F, Lawson DM, Tung Le* (2023). The CTP-binding domain is disengaged from the DNA-binding domain in a co-crystal structure of Bacillus subtilis Noc-DNA complex. J Biol Chem. 2023 Feb 23:103063. doi: 10.1016/j.jbc.2023.103063 (PDB: 7OL9)
McLean TC, Tung Le* (2023) CTP switches in ParABS-mediated bacterial chromosome segregation and beyond. Current Opinion in Microbiology 73:102289. doi:10.1016/j.mib.2023.102289
2022
Gozzi KR, Tran NT, Modell JW, Tung Le*, Laub MT*. (2022). Prophage-like gene transfer agents promote Caulobacter crescentus survival and DNA repair during stationary phase. PLOS Biology 2022, 20:e3001790.
Sukhoverkov KV, Jalal ASB, Lawson DM, Tung Le* (2022). The CTP-binding domain is disengaged from the DNA-binding domain in a co-crystal structure of Bacillus subtilis Noc-DNA complex. BioRxiv.2022.02.28.481274. doi:10.1101/2022.02.28.481274
Joseph A, Nahar K, Daw S, Hasan M, Lo R, Tung Le, Rahman K, Badrinarayanan A* (2022) Mechanistic insight into the repair of C8-linked
pyrrolobenzodiazepine monomer-mediated DNA damage. RSC Med Chem 2022. doi:10.1039/D2MD00194B. (preprint version)
2021
Szafran MJ, Malecki T, Strzalka A, Pawlikiewicz K, Dulawa J, Zarek A, Kois-Ostrowska A, Findlay K, Tung Le, Jakimowicz D*. (2021). Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development. Nature Communications 12:5222. doi:10.1038/s41467-021-25461-2 (preprint version)
Jalal, ASB, Tran, NT, Stevenson, CEM, Chimthanawala, A, Badrinarayanan, A, Lawson, DM, and Tung Le* (2021). A CTP-dependent gating mechanism enables ParB spreading on DNA. eLIFE 2021;10:e69676 (PDB: 6T1F and 7BM8) (GEO) (preprint version)
Jalal ASB, Tran NT, Wu LJ, Ramakrishnan K, Rejzek M, Gobbato G, Stevenson CEM, Lawson DM, Errington J, Tung Le* (2021). CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc. Molecular Cell doi:10.1016/j.molcel.2021.06.025 (PDB: 7NG0 and 7NFU) (preprint version)
Rejzek M* and Tung Le. (2021). Chemical synthesis and purification of a non-hydrolyzable CTP analog CTPɣS. BioRxiv 2021.01.13.426546. doi:10.1101/2021.01.13.426546 (preprint as final destination)
2020
Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Tung Le* (2020) Diversification of DNA-binding specificity via permissive and specificity-switching mutations in the ParB/Noc protein family. Cell Reports. 32(3):107928 (preprint version). (PDB:6S6H and 6Y93) (GEO)
Jalal ASB, Tung Le* (2020) Bacterial chromosome segregation by the ParABS system. Open Biology. 10: 200097.
Jalal ASB, Tran NT, Tung Le* (2020) ParB spreading on DNA requires cytidine triphosphate in vitro. eLIFE 9:e83515. (preprint version)
2019
Jalal ASB, Pastrana CL, Tran NT, Stevenson CE, Lawson DM, Moreno-Herrero F, Tung Le* (2019) Structural and biochemical analyses of Caulobacter crescentus ParB reveal the role of its N-terminal domain in chromosome segregation.BioRxiv. DOI. https://doi.org/10.1101/816959. (PDB: 6T1F) (GEO) (superseded by this version)
Tung Le* (2019) Chromosome conformation capture with deep sequencing to study the roles of the Structural Maintenance of Chromosomes (SMC) complex in vivo. Methods in Molecular Biology 2004:105-118. (free version)
2018
Schumacher MA, den Hengst CD, Bush MJ, Tung Le, Tran NT, Chandra G, Zeng W, Travis B, Brennan RG, Buttner MJ* (2018) The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.Nature Communications 9(1):1139-1151
Tran NT, Stevenson CE, Som NF, Thanapipatsiri A, Jalal ASB, Tung Le* (2018) Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome. Nucleic Acids Research. 46 (3), 1196–1209. (preprint version) (GEO)
2017
Tran NT, Laub MT, Tung Le* (2017) SMC progressively aligns chromosomal arms in Caulobacter crescentus but is antagonized by convergent transcription. Cell Reports 20(9):2057-2071 (preprint version) (GEO)
Badrinarayanan A, Tung Le, Spille JH, Cisse II, Laub MT*. (2017) Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB. PLoS Genetics. 13(5): e1006783. (GEO)
Wang X, Brandão HB, Tung Le, Laub MT, Rudner DZ* (2017) Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus. Science 355 (6324): 524-527
2016
Tung Le and Laub MT* (2016) Transcription rate and transcript length drive chromosomal interaction domain boundaries. EMBO Journal. 35(14):1582-95 (GEO)
2015
Wang X, Tung Le, Lajoie BR, Dekker J, Laub MT, Rudner DZ* (2015) Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtillus. Genes and Development 29(15):1661–75
Badrinarayanan A., Tung Le, Laub MT* (2015) Rapid pairing and subsequent resegregation of distant homologous loci enables double-strand break repair in bacteria. Journal of Cell Biology 210(3):385–400 (GEO)
Schäfer M, Tung Le, Hearnshaw SJ, Maxwell A, Challis GL, Wilkinson B, Buttner MJ* (2015) SimC7 Is a Novel NAD(P)H-Dependent Ketoreductase Essential for the Antibiotic Activity of the DNA Gyrase Inhibitor Simocyclinone. Journal of Molecular Biology. 427(12): 2192-204
Badrinarayanan A†, Tung Le†, Laub MT* (2015) Bacterial chromosome organization and segregation. Annual Review of Cell and Developmental Biology 31:171-99 († equal contribution)
2014
Tung Le and Laub MT* (2014) New approaches to understanding the spatial organization of bacterial genomes. Current Opinion in Microbiology 22:15-21
2013
Tung Le, Imakaev MV, Mirny LA, Laub MT* (2013) High-resolution mapping of the spatial organization of a bacterial chromosome. Science 342: 731-734 (GEO)
Stevenson CE, Assaad A, Chandra G, Tung Le, Greive SJ, Bibb MJ, Lawson DM* (2013) Investigation of DNA sequence recognition by a streptomycete MarR family transcriptional regulator through surface plasmon resonance and X-ray crystallography. Nucleic Acids Research 41: 7009-7022
2011
Tung Le*, Schumacher MA, Lawson DM, Brennan RG, Buttner MJ (2011) The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding. Nucleic Acids Research 39: 9433-9447.
Tung Le, Stevenson CE, Fiedler HP, Maxwell A, Lawson DM*, Buttner MJ (2011) Structures of the TetR-like simocyclinone efflux pump repressor, SimR, and the mechanism of ligand-mediated derepression. Journal of Molecular Biology 408: 40-56.
Tung Le, Stevenson CE, Buttner MJ, Lawson DM* (2011) Crystallization and preliminary X-ray analysis of the TetR-like efflux pump regulator SimR. Acta Crystallogr Sect F Struct Biol Cryst Commun 67: 307-309.
2009
Edwards MJ, Flatman RH, Mitchenall LA, Stevenson CE, Tung Le, Clarke TA, McKay AR, Fiedler HP, Buttner MJ, Lawson DM, Maxwell A* (2009) A crystal structure of the bifunctional antibiotic simocyclinone D8, bound to DNA gyrase. Science 326: 1415-1418.
Tung Le*, Fiedler HP, den Hengst CD, Ahn SK, Maxwell A, Buttner MJ (2009) Coupling of the biosynthesis and export of the DNA gyrase inhibitor simocyclinone in Streptomyces antibioticus. Mol Microbiol 72: 1462-1474.