*corresponding author

Lab members are highlited in bold

2020

Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Tung Le* (2020) Diversification of DNA-binding specificity via permissive and specificity-switching mutations in the ParB/Noc protein family. Cell Reports. 32(3):107928 (PREPRINT version). (PDB:6S6H and 6Y93) (GEO)

Jalal ASB, Tung Le* (2020) Bacterial chromosome segregation by the ParABS system. Open Biology. 10: 200097.

 

Jalal ASB, Tran NT, Tung Le* (2020) ParB spreading on DNA requires cytidine triphosphate in vitro. eLIFE 9:e83515. (PREPRINT version)

2019

​Jalal ASB, Pastrana CL, Tran NT, Stevenson CE, Lawson DM, Moreno-Herrero F, Tung Le (2019) Structural and biochemical analyses of Caulobacter crescentus ParB reveal the role of its N-terminal domain in chromosome segregation. PREPRINT. DOI. https://doi.org/10.1101/816959. (PDB: 6T1F) (GEO)

Tung Le* (2019) Chromosome conformation capture with deep sequencing to study the roles of the Structural Maintenance of Chromosomes (SMC) complex in vivo. Methods in Molecular Biology 2004:105-118. (FREE version)

2018

Schumacher MA, den Hengst CD, Bush MJ, Tung Le, Tran NT, Chandra G, Zeng W, Travis B, Brennan RG, Buttner MJ (2018) The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.Nature Communications 9(1):1139-1151

Tran NT, Stevenson CE, Som NF, Thanapipatsiri A, Jalal ASBTung Le* (2018) Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome. Nucleic Acids Research. 46 (3), 1196–1209. (PREPRINT version) (GEO)

2017

Tran NT, Laub MT, Tung Le* (2017) SMC progressively aligns chromosomal arms in Caulobacter crescentus but is antagonized by convergent transcription. Cell Reports 20(9):2057-2071 (PREPRINT version) (GEO)

Badrinarayanan A, Tung Le, Spille JH, Cisse II, Laub MT. (2017) Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB. PLoS Genetics. 13(5): e1006783. (GEO)

Wang X, Brandão HB, Tung Le, Laub MT, Rudner DZ (2017) Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus. Science 355 (6324): 524-527

2016

Tung Le and Laub MT (2016) Transcription rate and transcript length drive chromosomal interaction domain boundaries. EMBO Journal. 35(14):1582-95 (GEO)

2015

Wang X, Tung Le, Lajoie BR, Dekker J, Laub MT, Rudner DZ (2015) Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtillus. Genes and Development 29(15):1661–75

Badrinarayanan A., Tung Le, Laub MT (2015) Rapid pairing and subsequent resegregation of distant homologous loci enables double-strand break repair in bacteria. Journal of Cell Biology 210(3):385–400 (GEO)

Schäfer M, Tung Le, Hearnshaw SJ, Maxwell A, Challis GL, Wilkinson B, Buttner MJ (2015) SimC7 Is a Novel NAD(P)H-Dependent Ketoreductase Essential for the Antibiotic Activity of the DNA Gyrase Inhibitor Simocyclinone. Journal of Molecular Biology. 427(12): 2192-204

Badrinarayanan A†, Tung Le†, Laub MT (2015) Bacterial chromosome organization and segregation. Annual Review of Cell and Developmental Biology 31:171-99 († co-first)

2014

Tung Le and Laub MT (2014) New approaches to understanding the spatial organization of bacterial genomes. Current Opinion in Microbiology 22:15-21

2013

Tung Le, Imakaev MV, Mirny LA, Laub MT (2013) High-resolution mapping of the spatial organization of a bacterial chromosome. Science 342: 731-734 (GEO)

Stevenson CE, Assaad A, Chandra G, Tung Le, Greive SJ, Bibb MJ, Lawson DM (2013) Investigation of DNA sequence recognition by a streptomycete MarR family transcriptional regulator through surface plasmon resonance and X-ray crystallography. Nucleic Acids Research 41: 7009-7022

2011

Tung Le*, Schumacher MA, Lawson DM, Brennan RG, Buttner MJ (2011) The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding. Nucleic Acids Research 39: 9433-9447.

Tung Le, Stevenson CE, Fiedler HP, Maxwell A, Lawson DM, Buttner MJ (2011) Structures of the TetR-like simocyclinone efflux pump repressor, SimR, and the mechanism of ligand-mediated derepression. Journal of Molecular Biology 408: 40-56.

Tung Le, Stevenson CE, Buttner MJ, Lawson DM (2011) Crystallization and preliminary X-ray analysis of the TetR-like efflux pump regulator SimR. Acta Crystallogr Sect F Struct Biol Cryst Commun 67: 307-309.

2009

Edwards MJ, Flatman RH, Mitchenall LA, Stevenson CE, Tung Le, Clarke TA, McKay AR, Fiedler HP, Buttner MJ, Lawson DM, Maxwell A (2009) A crystal structure of the bifunctional antibiotic simocyclinone D8, bound to DNA gyrase. Science 326: 1415-1418.

Tung Le*, Fiedler HP, den Hengst CD, Ahn SK, Maxwell A, Buttner MJ (2009) Coupling of the biosynthesis and export of the DNA gyrase inhibitor simocyclinone in Streptomyces antibioticus. Mol Microbiol 72: 1462-1474.