*corresponding author

Lab members are highlited in bold

2022

Sukhoverkov KV, Jalal ASB, Lawson DM, Tung Le* (2022). The CTP-binding domain is disengaged from the DNA-binding domain in a co-crystal structure of Bacillus subtilis Noc-DNA complex. BioRxiv.2022.02.28.481274. doi:10.1101/2022.02.28.481274

Gozzi KR, Tran NT, Modell JW, Tung Le*, Laub MT*. (2022). Prophage-like gene transfer agents promote Caulobacter crescentus survival and DNA repair during stationary phase. PLOS Biology 2022, 20:e3001790.

Joseph A, Nahar K, Daw S, Hasan M, Lo R, Tung Le, Rahman K, Badrinarayanan A* (2022) Mechanistic insight into the repair of C8-linked
pyrrolobenzodiazepine monomer-mediated DNA damage
. RSC Med Chem 2022. doi:10.1039/D2MD00194B.
(preprint version)

2021

Szafran MJ, Malecki T, Strzalka A, Pawlikiewicz K, Dulawa J, Zarek A, Kois-Ostrowska A, Findlay K, Tung Le, Jakimowicz D*. (2021). Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development. Nature Communications 12:5222. doi:10.1038/s41467-021-25461-2 (preprint version)

Jalal, ASB, Tran, NT, Stevenson, CEM, Chimthanawala, A, Badrinarayanan, A, Lawson, DM, and Tung Le* (2021). A CTP-dependent gating mechanism enables ParB spreading on DNA. eLIFE 2021;10:e69676 (PDB: 6T1F and 7BM8) (GEO) (preprint version)

Jalal ASB, Tran NT, Wu LJ, Ramakrishnan K, Rejzek M, Gobbato G, Stevenson CEM, Lawson DM, Errington J, Tung Le* (2021). CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc. Molecular Cell doi:10.1016/j.molcel.2021.06.025 (PDB: 7NG0 and 7NFU) (preprint version)

Rejzek M* and Tung Le. (2021). Chemical synthesis and purification of a non-hydrolyzable CTP analog CTPɣS. BioRxiv 2021.01.13.426546. doi:10.1101/2021.01.13.426546 (preprint as final destination)

2020

Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Tung Le* (2020) Diversification of DNA-binding specificity via permissive and specificity-switching mutations in the ParB/Noc protein family. Cell Reports. 32(3):107928 (preprint version). (PDB:6S6H and 6Y93) (GEO)

Jalal ASB, Tung Le* (2020) Bacterial chromosome segregation by the ParABS system. Open Biology. 10: 200097.

 

​​Jalal ASB, Tran NT, Tung Le* (2020) ParB spreading on DNA requires cytidine triphosphate in vitro. eLIFE 9:e83515. (preprint version)

2019

​Jalal ASB, Pastrana CL, Tran NT, Stevenson CE, Lawson DM, Moreno-Herrero F, Tung Le* (2019) Structural and biochemical analyses of Caulobacter crescentus ParB reveal the role of its N-terminal domain in chromosome segregation.BioRxiv. DOI. https://doi.org/10.1101/816959. (PDB: 6T1F) (GEO) (superseded by this version)

Tung Le* (2019) Chromosome conformation capture with deep sequencing to study the roles of the Structural Maintenance of Chromosomes (SMC) complex in vivo. Methods in Molecular Biology 2004:105-118. (free version)

2018

Schumacher MA, den Hengst CD, Bush MJ, Tung Le, Tran NT, Chandra G, Zeng W, Travis B, Brennan RG, Buttner MJ* (2018) The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.Nature Communications 9(1):1139-1151

Tran NT, Stevenson CE, Som NF, Thanapipatsiri A, Jalal ASB, Tung Le* (2018) Permissive zones for the centromere-binding protein ParB on the Caulobacter crescentus chromosome. Nucleic Acids Research. 46 (3), 1196–1209. (preprint version) (GEO)

2017

Tran NT, Laub MT, Tung Le* (2017) SMC progressively aligns chromosomal arms in Caulobacter crescentus but is antagonized by convergent transcription. Cell Reports 20(9):2057-2071 (preprint version) (GEO)

Badrinarayanan A, Tung Le, Spille JH, Cisse II, Laub MT*. (2017) Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB. PLoS Genetics. 13(5): e1006783. (GEO)

Wang X, Brandão HB, Tung Le, Laub MT, Rudner DZ* (2017) Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus. Science 355 (6324): 524-527

2016

Tung Le and Laub MT* (2016) Transcription rate and transcript length drive chromosomal interaction domain boundaries. EMBO Journal. 35(14):1582-95 (GEO)

2015

Wang X, Tung Le, Lajoie BR, Dekker J, Laub MT, Rudner DZ* (2015) Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtillus. Genes and Development 29(15):1661–75

Badrinarayanan A., Tung Le, Laub MT* (2015) Rapid pairing and subsequent resegregation of distant homologous loci enables double-strand break repair in bacteria. Journal of Cell Biology 210(3):385–400 (GEO)

Schäfer M, Tung Le, Hearnshaw SJ, Maxwell A, Challis GL, Wilkinson B, Buttner MJ* (2015) SimC7 Is a Novel NAD(P)H-Dependent Ketoreductase Essential for the Antibiotic Activity of the DNA Gyrase Inhibitor Simocyclinone. Journal of Molecular Biology. 427(12): 2192-204

Badrinarayanan A†, Tung Le†, Laub MT* (2015) Bacterial chromosome organization and segregation. Annual Review of Cell and Developmental Biology 31:171-99 († equal contribution)

2014

Tung Le and Laub MT* (2014) New approaches to understanding the spatial organization of bacterial genomes. Current Opinion in Microbiology 22:15-21

2013

Tung Le, Imakaev MV, Mirny LA, Laub MT* (2013) High-resolution mapping of the spatial organization of a bacterial chromosome. Science 342: 731-734 (GEO)

Stevenson CE, Assaad A, Chandra G, Tung Le, Greive SJ, Bibb MJ, Lawson DM* (2013) Investigation of DNA sequence recognition by a streptomycete MarR family transcriptional regulator through surface plasmon resonance and X-ray crystallography. Nucleic Acids Research 41: 7009-7022

2011

Tung Le*, Schumacher MA, Lawson DM, Brennan RG, Buttner MJ (2011) The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding. Nucleic Acids Research 39: 9433-9447.

Tung Le, Stevenson CE, Fiedler HP, Maxwell A, Lawson DM*, Buttner MJ (2011) Structures of the TetR-like simocyclinone efflux pump repressor, SimR, and the mechanism of ligand-mediated derepression. Journal of Molecular Biology 408: 40-56.

Tung Le, Stevenson CE, Buttner MJ, Lawson DM* (2011) Crystallization and preliminary X-ray analysis of the TetR-like efflux pump regulator SimR. Acta Crystallogr Sect F Struct Biol Cryst Commun 67: 307-309.

2009

Edwards MJ, Flatman RH, Mitchenall LA, Stevenson CE, Tung Le, Clarke TA, McKay AR, Fiedler HP, Buttner MJ, Lawson DM, Maxwell A* (2009) A crystal structure of the bifunctional antibiotic simocyclinone D8, bound to DNA gyrase. Science 326: 1415-1418.

Tung Le*, Fiedler HP, den Hengst CD, Ahn SK, Maxwell A, Buttner MJ (2009) Coupling of the biosynthesis and export of the DNA gyrase inhibitor simocyclinone in Streptomyces antibioticus. Mol Microbiol 72: 1462-1474.