We say goodbye to Rebecca Lo, our PDRA; we wish her all the best in the next job in the NHS training program. Thank you for your hard work in our lab.

Our lab is back to work after the lock-down since 11th May, only 1 is allowed per bay. Progress is slow but spirit is high...

photo by Clare Stevenson.

Updated: Feb 21

Update: our preprint is now published at eLife (HERE)

A follow-up from great works by Gruber and Bange & Thanbichler labs: we show that Caulobacter ParB (and 8 other chromosomal ParB proteins) requires cytidine triphosphate (CTP) to spread in vitro: https://bit.ly/2rIO0Lh

We show that accumulation of Caulobacter ParB requires a closed DNA substrate with blocked ends and that a DNA-binding transcriptional regulator can act as a roadblock to attenuate spreading unidirectionally in vitro.

Our real-time and label-free in vitro reconstitution of Caulobacter ParB spreading has recapitulated many well-known aspects of ParB behaviors.

The ease and medium-throughput manner of our methodology hopefully will facilitate future works by the community to investigate many other aspects of ParB and bacterial chromosome segregation.

We thank the Gruber and Thanbichler groups for generously sharing bespoke reagents and pre-publication data.

Check out Gruber lab publication on Bacillus ParB-CTP story here: https://bit.ly/35qfN1P and Bange & Thanbichler lab publication on Myxococcus PadC and ParB-CTP story here: https://bit.ly/2Ph0ZMY